S-Adenosylhomocysteine (SAH) ELISA Kit
Product Sizes
96 assays
£897.00
MET-5151-96ASSAYS
About this Product
- SKU:
- MET-5151
- Application:
- ELISA
- Extra Details:
- S-adenosylhomocysteine (SAH) is generated through the donation of a methyl group from S-adenosylmethionine (SAM) to various biomolecules. SAH is then hydrolyzed to homocysteine and adenosine, which is converted to methionine, and ultimately back to SAM. SAH can act as a methylation inhibitor, therefore the SAM/SAH ratio, or methylation potential, is important for maintaining proper methylation in the cell. Our S-adenosylhomocysteine (SAH) ELISA is designed for detection and quantitation of SAH in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-SAH antibody are added to a plate coated with a SAH conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve. Samples with high SAH levels will bind the majority of the antibody, which gets washed away and results in a low OD, while samples with low SAH levels will leave more antibody available to bind to the conjugate, producing a high signal.
- Format:
- Colorimetric
- Physical State:
- Cell Lysate, Other Biological Fluid, Plasma, Serum, Tissue Lysate
- Research Area:
- Metabolism Research
- Sensitivity:
- 0.2 uM
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- Upon receipt, store SAH Conjugate and SAH Standard at -80ºC. Store the rest of the kit at 4ºC.
- Supplier:
- Cell BioLabs, Inc.
- Type:
- Assay Kits:ELISAs
- Detection Method:
- Colorimetric
