DAG (Diacylglycerol) Assay Kit
Product Sizes
100 assays
MET-5028-100ASSAYS
About this Product
- SKU:
- MET-5028
- Extra Details:
- Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit's Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.
- Format:
- Fluorometric
- Physical State:
- Cell Lysate
- Sensitivity:
- 4uM DAG
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -70[o]C Aliquot. Avoid freeze/thaw cycles. Protect from light.
- Supplier:
- CellBioLabs
- Type:
- Assay Kits: Detection
