Total Phosphatidic Acid Assay Kit (Fluorometric)
Product Sizes
100 assays
£660.00
MET-5019-100ASSAYS
About this Product
- SKU:
- MET-5019
- Extra Details:
- Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.
- Format:
- Fluorometric
- Research Area:
- Metabolism Research
- Sensitivity:
- approximately 5 uM phosphatidic acid
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
- Supplier:
- Cell BioLabs, Inc.
- Type:
- Assay Kits:Assays
- Detection Method:
- Fluorometric
