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Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons

Product Sizes
40 ml
TR-100-FJ-40ML
About this Product
SKU:
TR-100-FJ
Additional Names:
FJC
Application:
Flow Cytometry, IHC-Frozen, Immunocytochemistry, Immunohistochemistry
CE/IVD:
RUO
Concentration:
10X
Extra Details:
The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.
Format:
The reagents in the Fluoro Jade kit (10X) are all supplied in a liquid format and are ready-to-dilute., Fluorometric
Immunogen:
Degenerating neurons
Purification:
Purified
Reactivities:
Human, Mouse, Other Mammal, Rat
Shipping Conditions:
Blue Ice
Specificity:
Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain. Note: Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells). In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be pos
Storage Conditions:
Please refer to datasheet
Supplier:
Biosensis
Type:
Assay Kits: Staining & Imaging
Fluoro-Jade C (FJC) staining (green) of degenerating neurons in the CA3 region of the hippocampus in old diabetic rats. Blue: DAPI nuclear stain. Picture courtesy of Dr. Wang and colleagues. Results of this study were published in Wang S. et al. (2020), Geroscience, PMID: 32696219.
Double exposure using combined blue and ultraviolet epi-fluorescent illumination of the superficial layers of the cingulated rat cortex exposed to kainic acid. Layer I contains conspicuous Fluoro-Jade C positive degenerating axon terminals. Layer II contains densely packed DAPI-positive viable granule cells. Layer III contains a mixture of Fluoro-Jade C positive denegerating pyramidal cells and DAPI-positive viable pyramidal cells. Photo is courtesy of Dr. Larry Schmued.
Triple exposure combining ultraviolet, blue and green light epi-fluorescent illumination (10X) of rat hippocampus exposed to kainic acid. The section was triple labeled with Fluoro-Jade C and DAPI staining combined with GFAP immunohistochemistry. The section reveals extensive green Fluoro-Jade C positive neuronal degeneration throughout the entire CA-1 region of the hippocampus. The underlying blue viable positive granule cells of the dentate gyrus are only DAPI positive. Both regions exhibit red GFAP positive hypertrophied astrocytes. Photo is courtesy of Dr. Larry Schmued.