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Black-Gold II Myelin Ready-to-Dilute Staining Kit with Toluidine Blue O Counter Stain for identifying Normal & Pathogenic Myelin

Product Sizes
10 ml
TR-100-BG-10ML
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
TR-100-BG
Additional Names:
BlackGold, Black and Gold
Application:
IHC-Frozen, Immunohistochemistry
CE/IVD:
RUO
Concentration:
10X
Extra Details:
Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system. The Black Gold II Ready-to-Dilute (RTD) Staining Kit allows you to localise myelin, both individual fibres and tracts, along with the option of co-localising cell bodies via the Toluidine Blue counter stain. Black Gold II labelled myelinated fibres appear nearly black while the Toluidine Blue O labelled cellular Nissl bodies are blue under bright field illumination. Black Gold II can demonstrate and characterise specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid. Black Gold II can also be combined with other histochemical markers including Nissl stains, retrogradely transported fluorescent tracers and fluorescent markers of neuronal degeneration. The advantages associated with the Black-Gold II include high resolution, high contrast, short histochemical processing time, versatility and consistent reproducibility.
Format:
The reagents in the Black Gold kit (10X) are all supplied in a liquid format and are ready-to-dilute., Colorimetric
Immunogen:
Normal & pathogenic myelin
Purification:
Purified
Reactivities:
Other Mammal
Shipping Conditions:
Blue Ice
Specificity:
Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system.
Storage Conditions:
Please refer to datasheet
Size:
10 mL
Supplier:
Biosensis
Type:
Assay Kits: Staining & Imaging
Bright field illumination (60X mag) of the dentate gyrus of a normal mouse reveals individual Black-Gold II stained myelinated fibres and Toluadine Blue O stained polymorph cells (centre) and granule cells (top). Photo is courtesy of Dr. Larry Schmued.
Bright field illumination (60X mag) of 8 month old Ad-Tg mouse hippocampus. The myelin pathology can be observed in and around amyloid plaques. The cell bodies of adjacent granule and polymorph cells appear blue, while individual myeliated fibres appear nearly black. Photo is courtesy of Dr. Larry Schmued.
Human brain Locus K (Del Fiacco et al., Brain Struct Funct. 2014, 2019: 2083-2101; Cells 2018, 7, 72): calcitonin gene-related peptide-like immunoreactivity (black) and myelin staining (red, Black-Gold II staining kit, Biosensis) in the medulla oblongata, showing the similar neurochemical and structural organization of the dorsal column nuclei Locus K and the spinal trigeminal substantia gelatinosa.