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Assay Kits & ELISAs

A2 Beta casein (A2), Bovine, ELISA assay

Product Sizes
2 plate kit
£912.00
BEK244-2PLATEKIT
About this Product
SKU:
BEK244
Additional Names:
CSN2; Bovine A2 Beta Casein; A2
Application:
ELISA
CE/IVD:
RUO
Extra Details:
B Beta-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are B Beta-Casein A1 and B Beta-Casein A2. The two types of B Beta-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A2 B Beta-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A2 B Beta-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-bovine B Beta-Casein polyclonal capture antibody, a chicken anti-bovine A2 B Beta-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A2 B Beta-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A2 B Beta-Casein in full cream milk, skim milk and reconstituted A2 milk powder. Please refer to the kit protocol for specific use instructions. The A1 isoform is not detected in this ELISA assay.
Format:
Colorimetric
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A2
Physical State:
Milk
Range:
3.1 - 200 ng/mL
Reactivities:
Bovine
Sensitivity:
This bovine A2 beta-casein ELISA kit detects a minimum of 2 ng/mL A2 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10)).
Shipping Conditions:
Blue Ice
Specificity:
Bovine A2 beta-casein
Storage Conditions:
2-8[o]C
Supplier:
Biosensis
Type:
Assay Kits: ELISAs