A1 Beta casein (A1), Bovine, ELISA assay
Product Sizes
2 plate kit
£852.00
BEK243-2PLATEKIT
About this Product
- SKU:
- BEK243
- Additional Names:
- CSN2; Bovine A1 Beta Casein; A1
- Application:
- ELISA
- CE/IVD:
- RUO
- Extra Details:
- B Beta-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are B Beta-Casein A1 and B Beta-Casein A2. The two types of B Beta-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A1 B Beta-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A1 B Beta-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-Bovine B Beta-Casein polyclonal capture antibody, a chicken anti-bovine A1 B Beta-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A1 B Beta-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A1 B Beta-Casein in UHT (Ultra High Temperature) treated milk or long life milk, organic pasteurized and homogenized full cream milk, fresh pasteurized and homogenized milk, cold-pressed raw milk (non-pasteurized, non-homogenized), and biodynamic full-cream whole milk. Please refer to the kit protocol for specific use instructions. The A2 isoform is not detected in this ELISA assay.
- Format:
- Colorimetric
- Immunogen:
- Purified bovine beta Casein and peptide specific peptides for A1
- Physical State:
- Milk
- Range:
- 3.1 - 200 ng/mL
- Reactivities:
- Bovine
- Sensitivity:
- This bovine A1 beta-casein ELISA kit detects a minimum of 3 ng/mL A1 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10)).
- Shipping Conditions:
- Blue Ice
- Specificity:
- Bovine A1 beta-casein
- Storage Conditions:
- 2-8[o]C
- Supplier:
- Biosensis
- Type:
- Assay Kits: ELISAs
