LR3IGF-I, Human, Rapid[TM] ELISA assay
Product Sizes
2 plate kit
£852.00
BEK233-2PLATEKIT
About this Product
- SKU:
- BEK233
- Additional Names:
- Human LR3 insulin-like Growth Factor-I.
- Application:
- ELISA
- CE/IVD:
- RUO
- Extra Details:
- Human LR3 insulin-like Growth Factor-I (LR3IGF-I) was developed by GroPep Bioreagents (www.gropep.com) specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis. This LR3IGF-I Rapid ELISA kit combines GroPep's many years of expertise in the field of IGF research and Biosensis' newly established Rapid ELISA platform. This collaboration has resulted in the new LR3IGF-I Rapid ELISA kit, which provides for the sensitive, specific and reliable quantification of LR3IGF-I protein in less than 3 hours! The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR3IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR3IGF-I capture antibody pre-coated onto an ELISA plate, standard LR3IGF-I protein, a biotinylated anti- LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard. This LR3IGF-I Rapid ELISA kit has been developed, optimized and validated to quantify LR3IGF-I protein in cell culture medium. It is likely to be used to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Format:
- Colorimetric
- Immunogen:
- Recombinant LR3-IGF1 protein, made in E.coli
- Physical State:
- Cell Culture Supernatant
- Range:
- 3.9 ng/mL - 200 ng/mL
- Reactivities:
- Human
- Sensitivity:
- Typical limit of detection (LOD) for LR3IGF-I is 1 ng/mL determined as 150% of the blank value.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Human The assay is intended for quantification of LR3IGF-I. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
- Storage Conditions:
- 2-8[o]C
- Supplier:
- Biosensis
- Type:
- Assay Kits: ELISAs
