Glial cell line-derived neurotrophic factor (GDNF), Rat, Rapid[TM] ELISA assay
Product Sizes
1 plate kit
£482.00
BEK-2230-1PLATEKIT
About this Product
- SKU:
- BEK-2230
- Additional Names:
- Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor; ATF; GDNF
- Application:
- ELISA
- CE/IVD:
- RUO
- Extra Details:
- The Biosensis GDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant rat GDNF standard and is therefore designed to measure the rat form of GDNF. Note that the antibodies used in this kit cross-react with mouse, human and guinea pig GDNF. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Format:
- Colorimetric
- Immunogen:
- Recombinant rat GDNF protein, made in E.coli
- Physical State:
- Cell Culture Supernatant, Cell Lysate, Serum
- Range:
- 7.8 - 500 pg/mL
- Reactivities:
- Rat
- Sensitivity:
- Typical limit of detection (LOD) for rrGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
- Shipping Conditions:
- Blue Ice
- Specificity:
- Rat. The antibodies used in this kit detect human and mouse GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF RapidTM ELISA kit . All Biosensis GDNF RapidTM ELISA kits use the same capture and detection antibodies, and thus this Rat GDNF RapidTM ELISA kit can be used to assay guinea pig GDNF in serum. However, in a
- Storage Conditions:
- 2-8[o]C
- Supplier:
- Biosensis
- Type:
- Assay Kits: ELISAs
