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Assay Kits & ELISAs

Glial cell line-derived neurotrophic factor (GDNF), Mouse, Rapid[TM] ELISA assay

Product Sizes
1 plate kit
£482.00
BEK-2229-1PLATEKIT
About this Product
SKU:
BEK-2229
Additional Names:
Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor; ATF; GDNF
Application:
ELISA
CE/IVD:
RUO
Extra Details:
The Biosensis GDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant mouse GDNF standard and is therefore designed to measure the murine form of GDNF. Note that the antibodies used in this kit cross-react with rat, human and guinea pig GDNF. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Format:
Colorimetric
Immunogen:
Recombinant mouse GDNF protein, made in E.coli
Physical State:
Cell Culture Supernatant, Cell Lysate
Range:
7.8 - 500 pg/mL
Reactivities:
Mouse
Sensitivity:
Typical limit of detection (LOD) for rmGDNF is less than 5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Shipping Conditions:
Blue Ice
Specificity:
Mouse. The antibodies used in this kit detect human and rat GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF RapidTM ELISA kit . All Biosensis GDNF RapidTM ELISA kits use the same capture and detection antibodies, and thus this Mouse GDNF RapidTM ELISA kit can be used to assay guinea pig GDNF in serum. However, in
Storage Conditions:
2-8[o]C
Supplier:
Biosensis
Type:
Assay Kits: ELISAs