Mature NGF/proNGF Combo (BEK-2213/2236), Mouse, Rapid[TM] ELISA assay-2 x 1 Plate Set
Product Sizes
2 x 1 Plate Set
£614.00
BEK-2225-SET-2X1PLATESET
About this Product
- SKU:
- BEK-2225-SET
- Additional Names:
- Beta-nerve growth factor; Ngfb; NGF; pro-beta nerve growth factor; proNGF
- Application:
- ELISA
- Extra Details:
- The Biosensis Mouse Mature NGF/proNGF Combo RapidTM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important NGF isoforms: Mature NGF (BEK-2213) and full-length proNGF (BEK-2236).
Both kits are sandwich ELISAs, useful for the quantification of mature NGF and proNGF in less than 4 hours in cell culture supernatants and tissue homogenates only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Researchers have used both kits for NGF/proNGF measurement in mouse urine, however, Biosensis has not yet internally validated the use of urine in both ELISA kits. End-users are strongly advised to perform essential validation experiments to assure accurate NGF/proNGF quantification in mouse urine. Please refer to the most current kit protocols for BEK-2213 (Mature BDNF RapidTM ELISA) and BEK-2236 (proNGF RapidTM ELISA), for specific use instructions for each substrate application.
The Mature NGF ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proNGF ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature NGF or proNGF present in samples and protein standards. A NGF and proNGF positive control (QC sample) is provided to assure consistent assay performance.
The Mature NGF ELISA kit employs a native mouse NGF protein as standard, purified from mouse submaxillary glands according to published procedures. The calibrator standard reflects the native state of mouse NGF protein and has been chosen to give most accurate quantification of natural NGF protein levels in mouse samples. The proNGF ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli. Note that this Mature NGF/proNGF Combo kit is designed to measure the mouse protein forms, although due to sequence homology the Mature NGF ELISA kit will detect human and rat mature NGF. The proNGF ELISA kit cross-reacts with rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates and rat brain tissue homogenate. However, the proNGF ELISA shows only little cross-reactivity (20%) with human proNGF.
This Combo kit is capable of distinguishing and independently quantifying the mature NGF and full-length NGF isoforms. Internal validation data demonstrates <0.1% cross-reactivity of full-length mouse proNGF in the Mature NGF ELISA when assayed at 25 ng/mL in assay buffer, and proNGF is not detectable when assayed across the mature NGF calibration range. The antibodies used in the proNGF ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus the proNGF ELISA assay does not detect the pro-domain peptide. No cross-reactivity is observed in the proNGF ELISA kit with mature mouse NGF and full-length proBDNF when tested in assay buffer.
This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures. - Host:
- Mouse
- References:
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 2-8°C (on cold packs)
- Supplier:
- Biosensis