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Assay Kits & ELISAs

Apolipoprotein E/beta-Amyloid Complex (ApoE/A beta), Human, ELISA assay

Product Sizes
1 plate kit
£482.00
BEK-2224-1PLATEKIT
About this Product
SKU:
BEK-2224
Additional Names:
ApoE; Beta-APP42; Beta-APP40; Beta-amyloid protein 42; Beta-amyloid protein 40; ABPP; APPI; Amyloid beta A4 protein; MOAB2; MOAB-2; Alzheimer's antibody; AB40; AB42; abeta.
Application:
ELISA
CE/IVD:
RUO
Extra Details:
Biosensis is proud to offer the first commercially available ApoE/B Beta-amyloid (ApoE/AB Beta) complex ELISA kit. As a result of extensive collaboration with Dr. LaDu's laboratory at UIC and validation by Biosensis, this ELISA can be used to accurately and consistently measure the extent of ApoE/AB Beta complex in tissue extracts and other samples. The Biosensis ApoE/AB Beta Complex ELISA kit is a sandwich ELISA and consists of a pre-coated mouse monoclonal anti-AB Beta capture antibody, a highly validated ApoE/AB Beta complex standard that is pre-formed, lyophilized and ready for reconstitution, a biotinylated ApoE detection antibody, and horseradish peroxidase (HRP)-conjugated streptavidin and detection reagent. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the level of ApoE/AB Beta complex present in samples and protein standards. Importantly, a well-characterized and unique ApoE/AB Beta complex is included as a standard. This complex is pre-formed and lyophilized, requiring only reconstitution with assay diluent prior to use. In order to assess non-specific ApoE protein binding, each kit includes additional plates pre-coated with control antibody. The purpose of this kit is the in vitro qualitative measurement of ApoE/AB Beta complexes in brain extracts and CSF samples from both transgenic mice and humans or primates, relative to a known ApoE/AB Beta complex standard, only if used as directed. This kit has not been tested for other sample applications. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Format:
Colorimetric
Immunogen:
Complex of E.coli-derived recombinant human ApoE protein and synthetic, monomerized Abeta (1-42) peptide
Physical State:
Cerebrospinal Fluid, Tissue Homogenate
Range:
0.1 - 6.25%
Reactivities:
Human
Shipping Conditions:
Blue Ice
Specificity:
Human Apolipoprotein E/beta-Amyloid (ApoE/A beta) Complex. The kit has been assayed on human samples only but the capture antibody, MOAB-2, is know to react with rodent amyloid beta though weaker (20% less reactivity on dot blots). The polyclonal APOE used for detection should detect ApoE from a variety of species but so far has only been tested on human
Storage Conditions:
2-8[o]C
Supplier:
Biosensis
Type:
Assay Kits: ELISAs