Glial cell line-derived neurotrophic factor (GDNF), Human, Rapid[TM] ELISA assay
Product Sizes
1 plate kit
£482.00
BEK-2222-1PLATEKIT
About this Product
- SKU:
- BEK-2222
- Additional Names:
- Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor; ATF; GDNF
- Application:
- ELISA
- CE/IVD:
- RUO
- Extra Details:
- The Biosensis GDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and guinea pig serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant human GDNF standard obtained from the National Institute for Biological Standards and Control (NIBSC) and is therefore designed to accurately measure the human form of GDNF. Note that the antibodies used in this kit cross-react with guinea pig, rat and mouse GDNF. Guinea pig GDNF has been quantified in serum using the Human GDNF RapidTM ELISA kit, since guinea pig GDNF protein shares closest amino acid sequence homology with human GDNF. Protein levels can be expressed as human GDNF equivalents. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Format:
- Colorimetric
- Immunogen:
- Human recombinant GDNF with N-terminal methionine residue.
- Physical State:
- Cell Culture Supernatant, Cell Lysate, Serum
- Range:
- 7.8 - 500 pg/mL
- Reactivities:
- Human
- Sensitivity:
- Typical limit of detection (LOD) for rhGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
- Shipping Conditions:
- Blue Ice
- Specificity:
- Human. The antibodies used in this kit detect guinea pig, mouse and rat GDNF. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
- Storage Conditions:
- 2-8[o]C
- Supplier:
- Biosensis
- Type:
- Assay Kits: ELISAs
