Oligomeric Amyloid-beta, Human, Rat, ELISA assay
Product Sizes
1 plate kit
£482.00
BEK-2215-1PLATEKIT
About this Product
- SKU:
- BEK-2215
- Additional Names:
- Beta-APP42; Beta-APP40; Beta-amyloid protein 42; Beta-amyloid protein 40; ABPP; APPI; Amyloid beta A4 protein; MOAB2; MOAB-2; Alzheimer's antibody; AB40; AB42; abeta.
- Application:
- ELISA
- CE/IVD:
- RUO
- Extra Details:
- The oligomeric form of Amyloid Beta peptide (AB Beta, 1-42) has been closely linked to Alzheimer's Disease. Several ELISAs targeting AB Beta have been developed; however, these ELISAs are known to cross-react with Amyloid Beta precursor protein (APP) and are poorly characterized against monomeric and oligomeric forms of the peptide. The Biosensis MOAB-2 antibody, developed by LaDu and co-workers (Youmans K. et al., 2012), has been shown to specifically detect AB Beta, but not the precursor molecule APP. When utilized in ELISAs, the oligomeric form of AB Beta peptide (o-AB Beta) can be assayed independently of the other forms of the molecule when assayed with the MOAB-2 monoclonal antibody. The Biosensis oligomeric AB Beta ELISA kit is a sandwich ELISA that allows the preferential quantification of oligomeric AB Beta peptides. This kit is exclusive to Biosensis and consists of a pre-coated mouse monoclonal anti-AB Beta capture antibody (MOAB-2), a biotinylated MOAB-2 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of o-AB Beta present in samples and protein standards. The purpose of this kit is the in vitro qualitative measurement of oligomeric AB Beta peptide levels in brain extracts and CSF samples from both transgenic mice and humans relative to a known o-AB Beta standard. The inclusion of a highly validated oligomeric standard results in a unique, ready-to-use ELISA kit. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Format:
- Colorimetric
- Immunogen:
- The standard in this ELISA is synthetically manufactured beta-amyloid peptide, amino acids 1-42 of human, HFIP treated and dried.The stabilized oligomeric beta amyloid 1-42 control complex is also constructed from the same synthetic peptide standard material. No animal systems were used for their manufacture.
- Physical State:
- Cerebrospinal Fluid, Tissue Homogenate
- Range:
- 0.031 - 2 ng/mL
- Reactivities:
- Human, Rat
- Shipping Conditions:
- Blue Ice
- Specificity:
- Human. MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to AB Beta residues 1-4 and is highly specific just to amyloid beta peptide.The Biosensis o-AB Beta Elisa detects AB Beta oligomers as validated and described by Youmans KL et al (2012) and Rat by Combes M et al (2015). Rat.
- Storage Conditions:
- 2-8[o]C
- Supplier:
- Biosensis
- Type:
- Assay Kits: ELISAs
