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Proteins and Peptides

Dog Interleukin 6 (IL6), Active Protein

Product Sizes
50 ug
£974.00
RPU59252-50UG
100 ug
£1410.00
RPU59252-100UG
1 mg
£5905.00
RPU59252-1MG
About this Product
SKU:
RPU59252
Additional Names:
B-Cell Stimulatory Factor-2|BSF2|Cytotoxic T-Cell Differentiation Factor|Hepatocyte Stimulating Factor|HGF|HSF|Hybridoma/Plasmacytoma Growth Factor|IFNB2|MGI2-A|MGI2A
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Dog Interleukin 6 (IL6), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Interleukin 6 (IL6)
Molecular Weight:
25kDa
Physical State:
freeze-dried
Purity:
>95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Figure. Cell proliferation of Raji cells after stimulated with IL6.Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response and also plays a role in fighting infection. It supports the growth of B cells and is antagonistic to regulatory T cells. To test the effect of IL6 on cell proliferation, Raji cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 which contains various concentrations of recombinant dog IL6. After incubated for 5 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Raji cells after incubation with IL6 for 5 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant IL6 for 5 days. The result was shown in Figure 2. It was obvious that IL6 significantly increased cell viability of Raji cells.(A) Raji cells cultured in 1640, stimulated with 1ng/mL IL6 for 5 days; (B) Unstimulated Raji cells cultured in 1640 for 5 days.