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Proteins and Peptides

Human Spleen Tyrosine Kinase (SYK), Active Protein

Product Sizes
50 ug
£956.00
RPU59246-50UG
100 ug
£1383.00
RPU59246-100UG
1 mg
£5787.00
RPU59246-1MG
About this Product
SKU:
RPU59246
Additional Names:
p72-Syk|Spleen tyrosine kinase
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Spleen Tyrosine Kinase (SYK), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Spleen Tyrosine Kinase (SYK)
Molecular Weight:
34kda
Physical State:
freeze-dried
Purity:
>95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Figure. The binding activity of SYK with SIGLEC2.Spleen Tyrosine Kinase (SYK) is a member of the SYK family of tyrosine kinases. These non-receptor cytoplasmic tyrosine kinases share a characteristic dual SH2 domain separated by a linker domain. Within B and T cells respectively, SYK and Zap-70 transmit signals from the B-Cell receptor and T-Cell receptor. SYK plays a similar role in transmitting signals from a variety of cell surface receptors including CD74, Fc Receptor, and integrins. Besides, Sialic Acid Binding Ig Like Lectin 1 (SIGLEC2) has been identified as an interactor of SYK, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SYK and recombinant human SIGLEC2. Briefly, SYK were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SIGLEC2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-SYK pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of SYK and SIGLEC2 was shown in Figure 1, and this effect was in a dose dependent manner.