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Proteins and Peptides

Rat Surfactant Protein C (SP-C), Active Protein

Product Sizes
50 ug
£931.00
RPU59233-50UG
100 ug
£1346.00
RPU59233-100UG
1 mg
£5622.00
RPU59233-1MG
About this Product
SKU:
RPU59233
Additional Names:
PSP-C|Pulmonary Surfactant Protein C|Pulmonary surfactant-associated proteolipid SPL(Val)|SFTP2|SFTPC|SP5|SPC|Surfactant Associated Protein C
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Rat Surfactant Protein C (SP-C), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Surfactant Protein C (SP-C)
Molecular Weight:
14kDa
Physical State:
freeze-dried
Purity:
>95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Figure . Gene Sequencing (Extract)
Surfactant Associated Protein C (SPC) is one of the pulmonary surfactant proteins. It is a membrane protein which manufactures surfactant. The propeptide of pulmonary surfactant C has an N-terminal alpha-helical segment whose suggested function was stabilization of the protein structure, since the latter can irreversibly transform from its native alpha-helical structure to beta-sheet aggregates and form amyloid fibrils. Besides, Monokine Induced By Interferon Gamma (MIg) has been identified as an interactor of SPC, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat SPC and recombinant rat MIg. Briefly, SPC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MIg-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-SPC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of SPC and MIg was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of SPC with MIg.