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Proteins and Peptides

Human Transforming Growth Factor Beta 2 (TGFb2), Active Protein

Product Sizes
50 ug
£916.00
RPU59221-50UG
100 ug
£1324.00
RPU59221-100UG
1 mg
£5523.00
RPU59221-1MG
About this Product
SKU:
RPU59221
Additional Names:
BSC-1 cell growth inhibitor|Cetermin|G-TSF|Glioblastoma-derived T-cell suppressor factor|LAP|Latency-associated peptide|Polyergin|TGF-B2|TGF-Beta2
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Transforming Growth Factor Beta 2 (TGFb2), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Transforming Growth Factor Beta 2 (TGFb2)
Molecular Weight:
17kDa
Physical State:
freeze-dried
Purity:
>90%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Transforming growth factor beta (TGF-B Beta) is a multifunctional cytokine belonging to the transforming growth factor superfamily. The TGF-B Beta superfamily includes endogenous growth inhibiting proteins; an increase in expression of TGF-B Beta often correlates with the malignancy of many cancers and a defect in the cellular growth inhibition response to TGF-B Beta. Its immunosuppressive functions then come to dominate, contributing to oncogenesis. To test the effect of TGF-B Beta on inhibit HGF-dependent proliferation, HepG2 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1ng/mL HGF prior to the addition of various concentrations of recombinant human TGF-B Beta. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. The inhibitory effect of TGF-B Beta on HGF-dependent proliferation of HepG2 cells observed by inverted microscope was shown in Figure 2. Cell viability was assessed by CCK-8 assay after incubation with recombinant TGF-B Beta for 96h. The result was shown in Figure 3. It was obvious that TGF-B Beta significantly decreased cell viability of HepG2 cells. (A) HepG2 cells cultured in DMEM, stimulated with 1ug/mL TGF-B Beta for 96h; (B) Unstimulated HepG2 cells cultured in DMEM for 96h.Figure.The inhibitory effect of TGF-B Beta on cell proliferation of HepG2 cells.