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Proteins and Peptides

Human Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF), Active Protein

Product Sizes
50 ug
£877.00
RPU59156-50UG
100 ug
£1270.00
RPU59156-100UG
1 mg
£4957.00
RPU59156-1MG
About this Product
SKU:
RPU59156
Additional Names:
Arginine Rich Mutated In Early Stage Tumors|Arginine-rich protein|ARMET|ARP
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)
Molecular Weight:
22kDa
Physical State:
freeze-dried
Purity:
>90%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Figure . Gene Sequencing (Extract)
MANF (Mesencephalic astrocyte-derived neurotrophic factor) is a prosurvival protein that protects the cells when applied intracellularly in vitro or extracellularly in vivo. Recently, MANF has also been proven to inhibit cell proliferation and ER stress-induced cell death and also affects cell size and morphology under certain conditions. Thus the bioactivity of human recombinant MANF were detected using U87-MG. Briefly, U87-MG cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of MANF. After incubated for 48h, cells were observed by inverted microscope and were shown in Figure 1. It was obvious that MANF inhibited cell proliferation of U87-MG and affected cell size and morphology. (A)U7-MG cells cultured in DMEM, stimulated with 0.01ng/mL MANF for 48h; (B)Unstimulated U87-MG cells cultured in DMEM for 48h.Figure 1. Inhibition of U87-MG cell proliferation after stimulated with MANF.