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Proteins and Peptides

Human Interleukin 1 Alpha (IL1a), Active Protein

Product Sizes
50 ug
£820.00
RPU59102-50UG
100 ug
£1251.00
RPU59102-100UG
1 mg
£4602.00
RPU59102-1MG
About this Product
SKU:
RPU59102
Additional Names:
Hematopoietin-1|IL-1A Alpha|IL1-A|IL1F1|Interleukin-1 Family Member 1|Preinterleukin 1 Alpha|Pro-Interleukin-1-Alpha
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Interleukin 1 Alpha (IL1a), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Interleukin 1 Alpha (IL1a)
Molecular Weight:
24kDa
Physical State:
freeze-dried
Purity:
>95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Figure . SDS-PAGE
Figure. Cell proliferation of Jurkat cells after stimulated with IL1a.Interleukin 1 alpha (IL1A Alpha) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. IL1A Alpha is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha. To test the effect of IL1a on cell proliferation, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well with 2% serum standard 1640 including various concentrations of recombinant human IL1a. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Jurkat cells after incubation with IL1a for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant IL1a for 96h. The result was shown in Figure 2. It was obvious that IL1a significantly increased cell viability of Jurkat cells.(A) Jurkat cells cultured in 1640, stimulated with 10ng/mL IL1a for 96h; (B) Unstimulated Jurkat cells cultured in1640 for 96h.