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Proteins and Peptides

Human Ribonuclease P (RNASEP), Active Protein

Product Sizes
50 ug
£779.00
RPU59070-50UG
100 ug
£1186.00
RPU59070-100UG
1 mg
£4345.00
RPU59070-1MG
About this Product
SKU:
RPU59070
Additional Names:
Ribonuclease P/MRP 40kDa Subunit|Rnase-P|RNASEP1|RPP40
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Ribonuclease P (RNASEP), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Ribonuclease P (RNASEP)
Molecular Weight:
28kDa
Physical State:
freeze-dried
Purity:
>90%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Figure . Gene Sequencing (Extract)
Ribonuclease P (RNASEP) is a type of ribonuclease which cleaves RNA. RNase P is unique from other RNases in that it is a ribozyme - a ribonucleic acid that acts as a catalyst in the same way that a protein based enzyme would. Its function is to cleave off an extra, or precursor, sequence of RNA on tRNA molecules. Besides, Methyl CpG Binding Protein 2 (MECP2) has been identified as an interactor of RNASEP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human RNASEP and recombinant human MECP2. Briefly, RNASEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MECP2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-RNASEP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of RNASEP and MECP2 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of RNASEP with MECP2.