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Proteins and Peptides

Human Programmed Cell Death Protein 1 Ligand 1 (PDL1), Active Protein

Product Sizes
50 ug
£1008.00
RPU56361-50UG
100 ug
£1597.00
RPU56361-100UG
1 mg
£5997.00
RPU56361-1MG
About this Product
SKU:
RPU56361
Additional Names:
B7-H|B7H1|CD274|PD-L1|PDCD1-LG1|PDCD1L1|PDCD1LG1|Programed Death Ligand 1
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Programmed Cell Death Protein 1 Ligand 1 (PDL1), Active Protein is a purified Eukaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Programmed Cell Death Protein 1 Ligand 1 (PDL1)
Molecular Weight:
38kda
Physical State:
freeze-dried
Purity:
>97%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Besides, Programmed Cell Death Protein 1 (PDCD1) has been identified as an interactor of PDCD1LG1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PDCD1LG1 and recombinant human PDCD1. Briefly, PDCD1LG1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PDCD1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PDCD1LG1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PDCD1LG1 and PDCD1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of PDCD1LG1 with PDCD1.