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Proteins and Peptides

Mouse Matrix Metalloproteinase 2 (MMP2), Active Protein

Product Sizes
50 ug
£975.00
RPU56355-50UG
100 ug
£1543.00
RPU56355-100UG
1 mg
£5787.00
RPU56355-1MG
About this Product
SKU:
RPU56355
Additional Names:
72kDa Gelatinase|72kDa Type IV Collagenase|CLG4|CLG4A|Gelatinase A|MMP-II|MONA|PEX|TBE-1
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Mouse Matrix Metalloproteinase 2 (MMP2), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Matrix Metalloproteinase 2 (MMP2)
Molecular Weight:
63kDa
Physical State:
freeze-dried
Purity:
>95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Figure . SDS-PAGE
Mechanism: MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family .It is a gelatinase A, 72kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of MMP2 were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate-polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1 mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP2 protein as negative control, and blood sample as positive control .Result: Gelatin hydrolysis by recombinant mouse MMP2 was shown in figure 1.Figure 1. Hydrolysis of gelatin by recombinant mouse MMP2.