Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Proteins and Peptides

Mouse Tissue Inhibitors Of Metalloproteinase 3 (TIMP3), Active Protein

Product Sizes
50 ug
£878.00
RPU56338-50UG
100 ug
£1383.00
RPU56338-100UG
1 mg
£5155.00
RPU56338-1MG
About this Product
SKU:
RPU56338
Additional Names:
Metallopeptidase Inhibitor 3|Pseudoinflammatory|SFD|Sorsby Fundus Dystrophy|TIMP Metallopeptidase Inhibitor 3
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Mouse Tissue Inhibitors Of Metalloproteinase 3 (TIMP3), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)
Molecular Weight:
28/22kDa
Physical State:
freeze-dried
Purity:
>97%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Figure . Gene Sequencing (Extract)
Figure. The binding activity of TIMP3 with MMP2.Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) is an protein belongs to the tissue inhibitor of metalloproteinases family. They are inhibitors of the matrix metalloproteinases. TIMP-3 is the only member of the TIMP family which is found exclusively in the extracellular matrix (ECM). It is regulated in a cell cycle-dependent fashion in certain cell types and may serve as a marker for terminal differentiation. Besides, Matrix Metalloproteinase 2 (MMP2) has been identified as an interactor of TIMP3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse TIMP3 and recombinant mouse MMP2. Briefly, TIMP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to MMP2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TIMP3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TIMP3 and MMP2 was shown in Figure 1, and this effect was in a dose dependent manner.