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Proteins and Peptides

Human Caspase 3 (CASP3), Active Protein

Product Sizes
50 ug
£836.00
RPU56326-50UG
100 ug
£1015.00
RPU56326-100UG
1 mg
£4155.00
RPU56326-1MG
About this Product
SKU:
RPU56326
Additional Names:
Apoptain|Apoptosis-Related Cysteine Peptidase|CPP32|CPP32B|Cysteinyl Aspartate Specific Proteinases 3|SCA1|SREBP cleavage activity 1|Yama
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Caspase 3 (CASP3), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Caspase 3 (CASP3)
Molecular Weight:
14/18/20kDa
Physical State:
freeze-dried
Purity:
>90%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Figure . SDS-PAGE
Caspase 3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6 and 7; and the protein itself is processed and activated by caspases 8, 9, and 10. Caspase 3 can hydrolyze the peptide substrate acetyl-Asp-Glu-Val-Asp-p-nitroanilide (Ac-DEVD-pNA) resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405nm. Thus the activity of recombinant human caspase 3 can be measured by calculate the concentration of the pNA released from the substrate. The reaction was performed in adding 50uL 2xbuffer (50mM HEPES, 100mM NaCl, 10mM DTT, 2mM EDTA, 10% glycerol) to 96 well plates, then add 50uL various concentration of caspe 3 (diluted by 1xbuffer, 25mM HEPES, 50mM NaCl, 5mM DTT, 1mM EDTA, 5% glycerol) to each well, finally, add 5uL 4mmol Ac-DEVD-pNA to each well. Cover the 96 well plates and incubate at 37°C for 2h. p-Nitroaniline (pNA) standard curve prepare by double dilute 200uM pNA with 1xbuffer and record the OD value at 405nm. Calculate the caspase 3 activity in pmol of pNA released per min per ug recombinat human caspase 3.The specific activity of recombinant human caspase 3 is 2196pmol/min/ug.