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Proteins and Peptides

Human Superoxide Dismutase 3, Extracellular (SOD3), Active Protein

Product Sizes
50 ug
£638.00
RPU56290-50UG
100 ug
£791.00
RPU56290-100UG
1 mg
£3596.00
RPU56290-1MG
About this Product
SKU:
RPU56290
Additional Names:
EC-SOD|Extracellular Superoxide Dismutase Cu-Zn|SOD-3
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Superoxide Dismutase 3, Extracellular (SOD3), Active Protein is a purified Eukaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Superoxide Dismutase 3, Extracellular (SOD3)
Molecular Weight:
28kDa
Physical State:
freeze-dried
Purity:
>95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Figure . SDS-PAGE
Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris (hydroxymethyl) amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measure the activity of recombinant human SOD3. The reaction was performed in adding 30uL 50mmol/L pyrogallol to 900uL 50mmol/L Tris-HCl in 1.5ml cuvette (1.0cm light path), rapidly mixing at 25°C,then read at 325nm (zero the spectrophotometer using 50mmol/L Tris-HCl) , reacod the OD value every 30 second for 6 times. Control the pyrogallol autoxidation rate at 0.70 OD/min. After, adding various concentrations of recombinant of SOD3 to 900uL 50mmol/L Tris-HCl, incubated for 20min at 25°C, then adding 30uL 50mmol/L pyrogallol to each tube, rapidly mixing and read at 325nm, record the OD value every 30 second for 6 times. Under these conditions, the enzyme amount of 50% per minute inhibition of pyrogallol autooxidation is defined as a unit.