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Proteins and Peptides

Human Amphiregulin (AREG), Active Protein

Product Sizes
50 ug
£619.00
RPU56280-50UG
100 ug
£956.00
RPU56280-100UG
1 mg
£3470.00
RPU56280-1MG
About this Product
SKU:
RPU56280
Additional Names:
AR|Colorectum Cell-Derived Growth Factor|CRDGF|Schwannoma-Derived Growth Factor|SDGF
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Amphiregulin (AREG), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Amphiregulin (AREG)
Molecular Weight:
44kda
Physical State:
freeze-dried
Purity:
>90%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Figure . SDS-PAGE
Figure . Western Blot
Amphiregulin, also known as AREG, is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the Epidermal growth factor receptor (EGFR) to promote the growth of normal epithelial cells. Besides, Epidermal Growth Factor Receptor (EGFR) has been identified as an interactor of ARGE, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARGE and recombinant human EGFR. Briefly, ARGE were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EGFR-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ARGE pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ARGE and EGFR was shown in Figure 1, and this effect was in a dose dependent manner. Figure. The binding activity of ARGE with EGFR.