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Proteins and Peptides

Human Janus Kinase 2 (JAK2), Active Protein

Product Sizes
50 ug
£824.00
RPU54975-50UG
100 ug
£1001.00
RPU54975-100UG
1 mg
£4093.00
RPU54975-1MG
About this Product
SKU:
RPU54975
Additional Names:
JTK10|Tyrosine-protein kinase JAK2
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Janus Kinase 2 (JAK2), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Janus Kinase 2 (JAK2)
Molecular Weight:
35kda
Physical State:
freeze-dried
Purity:
>90%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
JAK2 (Tyrosine-protein kinase JAK2) is a tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. JAK2 is considered to associate with some type I receptors, including EPOR (Erythropoietin receptor), therefore participates in cellular signal transduction. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK2 and recombinant human EPOR. Briefly, JAK2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL JAK2 were then transferred to EPOR-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-JAK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of JAK2 and EPOR was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 3. SDS-PAGE
Figure 3. SDS-PAGE