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Proteins and Peptides

Human Janus Kinase 3 (JAK3), Active Protein

Product Sizes
50 ug
£1103.00
RPU54974-50UG
100 ug
£1348.00
RPU54974-100UG
1 mg
£5629.00
RPU54974-1MG
About this Product
SKU:
RPU54974
Additional Names:
A Protein Tyrosine Kinase,Leukocyte|JAKL|L-JAK|Leukocyte janus kinase|LJAK|Tyrosine-protein kinase JAK3
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Janus Kinase 3 (JAK3), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Janus Kinase 3 (JAK3)
Molecular Weight:
32kDa
Physical State:
freeze-dried
Purity:
>97%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
JAK3 (Tyrosine-protein kinase JAK3) is a non-receptor tyrosine kinase that involved in various processes such as cell growth, development, or differentiation. Besides, STAM2 has been identified as an interactor of JAK3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK3 and recombinant human STAM2. Briefly, JAK3 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAM2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-JAK3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of JAK3 and STAM2 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 3. SDS-PAGE
Figure 3. SDS-PAGE