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Proteins and Peptides

Human Lysyl Oxidase (LOX), Active Protein

Product Sizes
50 ug
£902.00
RPU54955-50UG
100 ug
£1098.00
RPU54955-100UG
1 mg
£4523.00
RPU54955-1MG
About this Product
SKU:
RPU54955
Additional Names:
Protein-Lysine 6-Oxidase
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Lysyl Oxidase (LOX), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Lysyl Oxidase (LOX)
Molecular Weight:
53kda
Physical State:
freeze-dried
Purity:
>90%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Lysyl oxidase (LOX) also known as protein-lysine 6-oxidase is an extracellular copper-dependent enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors. Its catalytic activity depends upon both its copper cofactor and a unique carbonyl cofactor and has been shown to extend to a variety of basic globular proteins, including histone H1. LOX plays a major role in connective tissue development and may also be important in neurological function. Lysyl oxidase has also proven crucial to the development of the respiratory system and the skin, the commitment step of adipocyte, and the formation of pluripotent stem cells during development. Its absence may lead to defects in the transforming growth factor beta superfamily of proteins, which control cell growth and differentiation. Besides, Elastin (ELN) has been identified as an interactor of LOX, thus a binding ELISA assay was conducted to detect the interaction of recombinant human LOX and recombinant human ELN. Briefly, LOX were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ELN-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-LOX pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of LOX and ELN was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 2. SDS-PAGE