Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Proteins and Peptides

Mouse Interleukin 6 Receptor (IL6R), Active Protein

Product Sizes
50 ug
£657.00
RPU54940-50UG
100 ug
£793.00
RPU54940-100UG
1 mg
£3172.00
RPU54940-1MG
About this Product
SKU:
RPU54940
Additional Names:
CD126|gp80|IL-6 receptor subunit alpha|IL-6R-1|IL-6R-Alpha|IL6R|IL6RA|Membrane glycoprotein 80
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Mouse Interleukin 6 Receptor (IL6R), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Interleukin 6 Receptor (IL6R)
Molecular Weight:
18kDa
Physical State:
freeze-dried
Purity:
>97%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
IL6R (Interleukin-6 receptor subunit alpha) is part of the receptor for interleukin 6. The interaction of IL6R and IL6 may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Thus, a binding ELISA assay was conducted to detect the association of IL6R with IL6. Briefly, IL6R were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL6-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL6R pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL6R with IL6 was shown in Figure 1 and this effect was in a dose dependent manner.
Figure 2. SDS-PAGE