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Proteins and Peptides

Human Interleukin 10 Receptor Beta (IL10Rb), Active Protein

Product Sizes
50 ug
£691.00
RPU54932-50UG
100 ug
£836.00
RPU54932-100UG
1 mg
£3356.00
RPU54932-1MG
About this Product
SKU:
RPU54932
Additional Names:
CD210-B|CDW210b|CRF2-4|CRFB4|IL-10R2|IL10-RB|IL10R-B|Interleukin-10 receptor subunit 2
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Interleukin 10 Receptor Beta (IL10Rb), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Interleukin 10 Receptor Beta (IL10Rb)
Molecular Weight:
18kDa
Physical State:
freeze-dried
Purity:
>97%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
IL10Rb (Interleukin-10 receptor subunit beta) belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex, which is a cell surface receptor required for the activation of several cytokines, including IL10. Thus, a binding ELISA assay was conducted to detect the interaction of IL10Rb and IL10. Briefly, recombinant human IL10RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL10Rb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL10Rb and IL10 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 2. SDS-PAGE