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Proteins and Peptides

Human Indoleamine-2,3-Dioxygenase (IDO), Active Protein

Product Sizes
50 ug
£936.00
RPU54927-50UG
100 ug
£1140.00
RPU54927-100UG
1 mg
£4707.00
RPU54927-1MG
About this Product
SKU:
RPU54927
Additional Names:
CD107B|INDO|Indoleamine-Pyrrole 2,3 Dioxygenase
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Indoleamine-2,3-Dioxygenase (IDO), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Indoleamine-2,3-Dioxygenase (IDO)
Molecular Weight:
43kDa
Physical State:
freeze-dried
Purity:
>97%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Active Indoleamine-2,3-Dioxygenase (IDO), RPU54927
IDO (Indoleamine 2,3-dioxygenase 1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. Thus, bioactivity of recombinant human IDO was measured through its ability to oxidize L-tryptophan to N-formyl-kynurenine, using Methylene Blue as indicator. Briefly, a mixture of 800uM L-tryptophan, 9000 units/mL catalase, and 40uM Methyene Blue with the addition of 80mM ascorbic acid reaction buffer was incubated with different concentrations of IDO, and the absorbance was read in 321nm every 1.5h. The result indicated that recombinant human IDO oxidize L-tryptophan.
Figure 3. SDS-PAGE