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Proteins and Peptides

Human Death receptor 5 (DR5), Active Protein

Product Sizes
50 ug
£847.00
RPU54910-50UG
100 ug
£1029.00
RPU54910-100UG
1 mg
£4216.00
RPU54910-1MG
About this Product
SKU:
RPU54910
Additional Names:
CD262|Death receptor 5|KILLER|TNF-related apoptosis-inducing ligand receptor 2|TNFRSF10B|TRAILR2|TRICK2|TRICK2A|TRICK2B|TRICKB|Tumor Necrosis Factor Receptor Superfamily, Member 10B|ZTNFR9
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Death receptor 5 (DR5), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Death receptor 5 (DR5)
Molecular Weight:
53kda
Physical State:
freeze-dried
Purity:
>97%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
TNFRSF10B (Tumor necrosis factor receptor superfamily member 10B) is a member of the TNF-receptor superfamily, and contains an intracellular death domain. By binding to certain ligand, this receptor transduces an apoptosis signal. A binding ELISA assay was conducted to detect the association of FAS with TNFa. Briefly, FAS were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL FAS were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-FAS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FAS and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 2. SDS-PAGE