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Proteins and Peptides

Human Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A), Active Protein

Product Sizes
50 ug
£1081.00
RPU54909-50UG
100 ug
£1321.00
RPU54909-100UG
1 mg
£5506.00
RPU54909-1MG
About this Product
SKU:
RPU54909
Additional Names:
CD266|Fibroblast growth factor-inducible immediate-early response protein 14|FN14|TNFRSF12-A|Tumour Necrosis Factor Related Weak Inducer Of Apoptosis|TWEAKR
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A)
Molecular Weight:
44kda
Physical State:
freeze-dried
Purity:
>80%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 2. SDS-PAGE