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Proteins and Peptides

Rat Glutathione S Transferase Mu 1 (GSTM1), Active Protein

Product Sizes
50 ug
£1147.00
RPU54902-50UG
100 ug
£1404.00
RPU54902-100UG
1 mg
£5874.00
RPU54902-1MG
About this Product
SKU:
RPU54902
Additional Names:
GST HB subunit 4|GST-M1|GST1|GSTM1-1|GSTM1a-1a|GSTM1b-1b|GTH4|GTM1|H-B|MU1
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Rat Glutathione S Transferase Mu 1 (GSTM1), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Glutathione S Transferase Mu 1 (GSTM1)
Molecular Weight:
27kDa
Physical State:
freeze-dried
Purity:
>95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Glutathione S-transferase Mu 1 (GSTM1) belongs to the mu class. GSTM1 can detoxifying carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Besides, uncoupling Protein 1 (UCP1) has been identified as an interactor of GSTM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GSTM1 and recombinant rat UCP1. Briefly, GSTM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCP1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GSTM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GSTM1 and UCP1 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 2. SDS-PAGE