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Proteins and Peptides

Mouse S100 Calcium Binding Protein B (S100B), Active Protein

Product Sizes
50 ug
£657.00
RPU54900-50UG
100 ug
£793.00
RPU54900-100UG
1 mg
£3172.00
RPU54900-1MG
About this Product
SKU:
RPU54900
Additional Names:
NEF|S-100 protein subunit beta|S100-B|S100Beta
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Mouse S100 Calcium Binding Protein B (S100B), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
S100 Calcium Binding Protein B (S100B)
Molecular Weight:
10kDa
Physical State:
freeze-dried
Purity:
>90%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Besides, mouse RAGE shares similarities with human RAGE in amino acids sequence with the identity of 78.2%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 2. SDS-PAGE