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Proteins and Peptides

Human Granzyme M (GZMM), Active Protein

Product Sizes
50 ug
£991.00
RPU54892-50UG
100 ug
£1209.00
RPU54892-100UG
1 mg
£5014.00
RPU54892-1MG
About this Product
SKU:
RPU54892
Additional Names:
GZM-M|Hu-Met-1|LMET1|Lymphocyte Met Ase 1|Met-1 serine protease|Met-ase|MET1|Natural killer cell granular protease
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Granzyme M (GZMM), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Granzyme M (GZMM)
Molecular Weight:
26kDa
Physical State:
freeze-dried
Purity:
>90%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets A Alpha-Tubulin and disorganizes the microtubule neTwork, besides, Ezrin has also been identified as a substrate of GZMM. Therefore, a catalytic assay was conducted to detect the protease activity of recombinant human GZMM using Hela cells lysates. Briefly, protein lysates were extracted from 2x107 Hela cells using Lysis Buffer, then incubated with normal or inactivated GZMM in 37oC for 4h. Samples were immunoblotted using Abs B Beta-actin as control, and Ezrin to detect the enzyme activity. The results were shown below. It is obvious that recombinant human GZMM cleaved Ezrin.
Figure 3. SDS-PAGE
Figure 3. SDS-PAGE