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Proteins and Peptides

Human Growth Differentiation Factor 9 (GDF9), Active Protein

Product Sizes
50 ug
£1016.00
RPU54891-50UG
100 ug
£1240.00
RPU54891-100UG
1 mg
£5149.00
RPU54891-1MG
About this Product
SKU:
RPU54891
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Growth Differentiation Factor 9 (GDF9), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Growth Differentiation Factor 9 (GDF9)
Molecular Weight:
18kDa
Physical State:
freeze-dried
Purity:
>90%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
GDF9 (Growth/differentiation factor 9) is an oocyte derived growth factora which belongs to the transforming growth factor-beta (TGFB Beta) superfamily. GDF9 is required for ovarian folliculogenesis and promotes primordial follicle development. S100A8 has been identified as an interactor of GDF9 through Two-hybrid assay, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GDF9 and recombinant human S100A8. Briefly, GDF9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100A8-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GDF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GDF9 and S100A8 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 2. SDS-PAGE