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Proteins and Peptides

Mouse Hexosaminidase A Alpha (HEXa), Active Protein

Product Sizes
50 ug
£1031.00
RPU54881-50UG
100 ug
£1258.00
RPU54881-100UG
1 mg
£5229.00
RPU54881-1MG
About this Product
SKU:
RPU54881
Additional Names:
Beta-Hexosaminidase Subunit Alpha|Beta-N-Acetylhexosaminidase Subunit Alpha|GM2 Gangliosidosis|Hex-A|N-Acetyl-Beta-Glucosaminidase Alpha|Tay Sachs Disease|TSD
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Mouse Hexosaminidase A Alpha (HEXa), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Hexosaminidase A Alpha (HEXa)
Molecular Weight:
28kDa
Physical State:
freeze-dried
Purity:
>97%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Hexosaminidase A Alpha (HEXa) is a lysosomal enzyme. There are three predominant isoenzymes: hexosaminidase A, B and S. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines. The enzymes are composed of Two alpha and/or beta subunits, which are coded by HEXA and HEXB genes, respectively. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit which contains a key residue, Arg-424 is able to hydrolyze GM2 gangliosides. Hexosaminidase A (alpha polypeptide) plays a critical role in the brain and spinal cord (central nervous system). Besides, Hexosaminidase B Beta (HEXB) has been identified as an interactor of HEXA, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse HEXA and recombinant mouse HEXB. Briefly, HEXA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HEXB-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-HEXA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of HEXA and HEXB was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 2. SDS-PAGE