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Proteins and Peptides

Human Leukemia Inhibitory Factor (LIF), Active Protein

Product Sizes
50 ug
£606.00
RPU54869-50UG
100 ug
£749.00
RPU54869-100UG
1 mg
£3385.00
RPU54869-1MG
About this Product
SKU:
RPU54869
Additional Names:
CDF|Cholinergic Differentiation Factor|D-FACTOR|Differentiation-Stimulating Factor|Emfilermin|HILDA|Melanoma-Derived LPL Inhibitor|MLPLI
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Leukemia Inhibitory Factor (LIF), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Leukemia Inhibitory Factor (LIF)
Molecular Weight:
26kDa
Physical State:
freeze-dried
Purity:
>95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
Leukemia inhibitory factor (LIF) is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 1x104 cells/well and incubated for 72h in the presence or absence of various concentrations of LIF at 37°C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 °C. Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1.
The dose-effect curve of LIF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 0.05 to 1.67ng/mL