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Proteins and Peptides

Human Factor Related Apoptosis (FAS), Active Protein

Product Sizes
50 ug
£1069.00
RPU54848-50UG
100 ug
£1306.00
RPU54848-100UG
1 mg
£5444.00
RPU54848-1MG
About this Product
SKU:
RPU54848
Additional Names:
ALPS1-A|ALPS1A|APO1|APT1|CD95|Fas Receptor|FAS1|FASTM|TNF Receptor Superfamily Member 6|TNFRSF6|Tumor Necrosis Factor Receptor Superfamily Member 6
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Factor Related Apoptosis (FAS), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Factor Related Apoptosis (FAS)
Molecular Weight:
66kDa
Physical State:
freeze-dried
Purity:
>95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
FAS (Tumor necrosis factor receptor superfamily member 6) belongs to the tumor necrosis factor receptor superfamily. FAS contains a death domain, which has been shown to play a central role in the physiological regulation of programmed cell death. A binding ELISA assay was conducted to detect the association of FAS with TNFa. Briefly, FAS were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL FAS were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-FAS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FAS and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.
Figure 3. SDS-PAGE
Figure 3. SDS-PAGE