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Proteins and Peptides

Human Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF), Active Protein

Product Sizes
50 ug
£684.00
RPU54823-50UG
100 ug
£1062.00
RPU54823-100UG
1 mg
£3891.00
RPU54823-1MG
About this Product
SKU:
RPU54823
Additional Names:
CSF2|GMCSF|Granulocyte-Macrophage Colony Stimulating Factor|Molgramostin|Sargramostim
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF), Active Protein is a purified Eukaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
Molecular Weight:
19-26kDa
Physical State:
freeze-dried
Purity:
>95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
TF-1, the human erythroleukemia cell line,provides a good system for detecting the activity of GM-CSF for it is a cell line of immature erythroid origin that completely depends on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. As reported, GM-CSF was also able to induce differentiation of human monoblastic leukemia cell line U937. In house data was obtained by the following experiment: TF-1 cells and U937 cells were incubated in the presence of various concentrations of rhGM-CSF, then cells were observed by inverted microscope everyday . Cell proliferation of TF1 cells after incubation with GM-CSF (10ng/mL) for 3 days was shown in Figure 1.
Cell differentiation of U937 cells after incubation with GM-CSF (10ng/mL) for 5 days was shown in Figure 2.
Figure 3. SDS-PAGE