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Proteins and Peptides

Human Fibronectin Type III Domain Containing Protein 5 (FNDC5), Active Protein

Product Sizes
50 ug
£853.00
RPU54776-50UG
100 ug
£1036.00
RPU54776-100UG
1 mg
£4247.00
RPU54776-1MG
About this Product
SKU:
RPU54776
Additional Names:
Fibronectin type III repeat-containing protein 2|FRCP2|Irisin
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Fibronectin Type III Domain Containing Protein 5 (FNDC5), Active Protein is a purified Eukaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Fibronectin Type III Domain Containing Protein 5 (FNDC5)
Molecular Weight:
27kDa
Physical State:
freeze-dried
Purity:
>97%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Catalog Protein.JPG
The protein encoded by FNDC5 can be cleaved into Irisin, which is a myokine linked to exercise and lean body mass. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours in at 37oC. Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope was shown in Figure 1.
Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of FNDC5 for 48h. The dose-effect curve of FNDC5 was shown in Figure 2. It was obvious that FNDC5 significantly decreased cell viability of MDA-MB-231 cells. The ED50 for this effect is typically 1.875 nM.