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Proteins and Peptides

Human Lemur Tyrosine Kinase 3 (LMTK3), Active Protein

Product Sizes
50 ug
£853.00
RPU54775-50UG
100 ug
£1036.00
RPU54775-100UG
1 mg
£4247.00
RPU54775-1MG
About this Product
SKU:
RPU54775
Additional Names:
LMR3|Serine/threonine-protein kinase LMTK3|TYKLM3
Application:
Detection/Quantitation, Tissue Culture
Buffer:
Freeze-dried powder|PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
CE/IVD:
RUO
Extra Details:
Human Lemur Tyrosine Kinase 3 (LMTK3), Active Protein is a purified Prokaryotic Protein.
Formulation:
Freeze-dried powder
Immunogen:
Lemur Tyrosine Kinase 3 (LMTK3)
Molecular Weight:
46kda
Physical State:
freeze-dried
Purity:
>95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Catalog Protein.JPG
Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of LMTK3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.
The dose-effect curve of LMTK3 was shown in Figure 2. It was obvious that LMTK3 significantly promoted cell proliferation of MCF-7 cells. The ED50 for this effect is approximately 6.14~50ng/mL.
Figure 3. SDS-PAGE