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Antibodies

Acetyl-Hist1H4A (K31) Polyclonal Antibody

Product Sizes
50 ul
CAC11540-50UL
100 ul
CAC11540-100UL
About this Product
SKU:
CAC11540
Additional Names:
dJ160A22.1|dJ160A22.2|dJ221C16.1|dJ221C16.9|FO108|H4|H4_HUMAN|H4.k|H4/a|H4/b|H4/c|H4/d|H4/e|H4/g|H4/h|H4/I|H4/j|H4/k|H4/m|H4/n|H4/p|H4F2|H4F2iii|H4F2iv|H4FA|H4FB|H4FC|H4FD|H4FE|H4FG|H4FH|H4FI|H4FJ|H4FK|H4FM|H4FN|H4M|HIST1H4A|HIST1H4B|HIST1H4C|HIST1H4D|HIST1H4E|HIST1H4F|HIST1H4H|HIST1H4I|HIST1H4J|HIST1H4K|HIST1H4L|HIST2H4|HIST2H4A|Hist4h4|Histone 1 H4a|Histone 1 H4b|Histone 1 H4c|Histone 1 H4d|Histone 1 H4e|Histone 1 H4f|Histone 1 H4h|Histone 1 H4i|Histone 1 H4j|Histone 1 H4k|Histone 1 H4l|Histone 2 H4a|histone 4 H4|Histone H4|MGC24116
Application:
ChIP, ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Acetyl-Hist1H4A (K31) Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, ICC, IF, ChIP.
Host:
Rabbit
Immunogen:
HIST1H4A
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Western Blot; Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H4A antibody at 1:100; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 12 kDa; Observed band size: 12 kDa
Immunocytochemistry analysis diluted at 1:25 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Chromatin Immunoprecipitation Hela (106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A (CAC11540) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the B Beta-Globin promoter.