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Antibodies

Hecw2 Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC11479-50UG
100 ug
£454.00
CAC11479-100UG
About this Product
SKU:
CAC11479
Additional Names:
C2 and WW domain containing E3 ubiquitin protein ligase 2|C2 and WW domain-containing protein 2|E3 ubiquitin protein ligase HECW2|E3 ubiquitin-protein ligase HECW2|HECT|Hecw2|HECW2_HUMAN|KIAA130|KIAA1301|NEDD4 like E3 ubiquitin protein ligase 2|NEDD4 Related E3 Ubiquitin Ligase|NEDD4-like E3 ubiquitin-protein ligase 2
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Hecw2 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
HECW2
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate; All lanes: HECW2 antibody at 3.5ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 176, 136 kDa; Observed band size: 176 kDa
IHC image diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CAC11479 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).