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Antibodies

Alox12 Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC11434-50UG
100 ug
£454.00
CAC11434-100UG
About this Product
SKU:
CAC11434
Additional Names:
12 LOX|12-lipoxygenase|12(S) lipoxygenase|12LO|12S LOX|12S type|12S-lipoxygenase|12S-LOX|12S-type|Alox12|Arachidonate 12 lipoxygenase|Arachidonate 12-lipoxygenase|Arachidonate 12-oxidoreductase|Lipoxin synthase 12-LO|LOG12|LOX12_HUMAN|P-12LO|Platelet 12-LOX|Platelet type lipoxygenase 12|platelet-type 12-lipoxygenase|Platelet-type lipoxygenase 12
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Alox12 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
ALOX12
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate; All lanes: ALOX12 antibody at 3.53ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 76 kDa; Observed band size: 76 kDa
IHC image diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CAC11434 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).