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Antibodies

Elp3 Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC11360-50UG
100 ug
£454.00
CAC11360-100UG
About this Product
SKU:
CAC11360
Additional Names:
DKFZp761L098|ELONGATION PROTEIN 3|Elongation protein 3 homolog|Elongation protein 3 homolog (S. cerevisiae)|elongator acetyltransferase complex subunit 3|Elongator complex protein 3|elp3|ELP3_HUMAN|FLJ10422|hELP3|HOMOLOG OF|Hypothetical protein DKFZp761L098|Kat9|S. CEREVISIAE
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Elp3 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF, IP.
Host:
Rabbit
Immunogen:
ELP3
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, 293 whole cell lysate; All lanes: ELP3 antibody at 3.82ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 63, 61, 50, 53 kDa; Observed band size: 63 kDa
IHC image diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC11360 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating ELP3 in HEK293 whole cell lysate; , Lane 1: Rabbit control IgG instead in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11360 (6ug) + HEK293 whole cell lysate (500ug); , Lane 3: HEK293 whole cell lysate (20ug)