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Antibodies

Epcam Polyclonal Antibody

Product Sizes
50 ug
£302.00
CAC11319-50UG
100 ug
£454.00
CAC11319-100UG
About this Product
SKU:
CAC11319
Additional Names:
17 1A|323/A3|35-KD glycoprotein|Adenocarcinoma associated antigen|Adenocarcinoma-associated antigen|Antigen identified by monoclonal AUA1|AUA1|CD326|CD326 antigen|Cell surface glycoprotein Trop 1|Cell surface glycoprotein Trop 2|Cell surface glycoprotein Trop-1|chromosome 4|CO 17A|CO17 1A|CO17A|DIAR5|EGP|EGP 2|EGP2|EGP314|EGP40|Ep CAM|Ep-CAM|EPCAM|EPCAM_HUMAN|EpCAM1|Epithelial cell adhesion molecule|Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD)|Epithelial cell surface antigen|Epithelial cellular adhesion molecule|Epithelial glycoprotein|Epithelial glycoprotein 1|Epithelial glycoprotein 314|ESA|GA733 1|GA733 2|GA733-2|gastrointestinal tumor-associated antigen 2|gp4|hEGP 2|hEGP314|HNPCC8|Human epithelial glycoprotein 2|KS 1/4 antigen|KS1/4|KSA|Ly74|Lymphocyte antigen 74|M1S 1|M1S2|M4S1|Major gastrointestinal tumor associated protein GA733 2|Major gastrointestinal tumor-associated protein GA733-2|mEGP314|Membrane component|Membrane component chromosome 4 surface marker (35kD glycoprotein)|MIC18|MK 1|Protein 289A|surface marker|surface marker 1|TACD1|TACSTD1|TROP1|Tumor associated calcium signal transducer 1|Tumor associated calcium signal transducer 2 precursor|Tumor-associated calcium signal transducer 1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01m PBS
CE/IVD:
RUO
translate.label.attr.clone:
Polyclonal
Clonality:
Polyclonal
Extra Details:
The Epcam Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Host:
Rabbit
Immunogen:
EPCAM
Isotype:
IgG
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Rat
Shipping Conditions:
Blue Ice
Source:
Rabbit
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Polyclonal Antibody
Catalog Antibody.JPG
Western Blot; Positive WB detected in: Rat liver tissue; All lanes: EPCAM antibody at 9.6ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 35 kDa; Observed band size: 35 kDa
IHC image diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with CAC11319 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).